Diving into the Genetic Pool and Molecular Treasury of Sphagneticola trilobata: A Unique Journey with ISSR Markers

The research aimed to explore the genetic diversity of Sphagneticola trilobata populations sourced from Thiruvananthapuram and Alleppey, two distinct locations in Kerala, India, utilizing ISSR (Inter Simple Sequence Repeat) markers, a robust tool in population genetics for assessing genetic variation. Subsequently, DNA extraction was carried out following established molecular biology protocols to obtain high-quality genomic DNA from the collected samples. ISSR analysis was then conducted, employing PCR amplification targeting regions between microsatellite sequences, with primers designed to anneal at these sites. The resulting amplification patterns served as fingerprints, reflecting the genetic variation within the samples. For comprehensive data analysis, Nei's gene diversity and Shannon's Information index were computed to quantitatively assess genetic diversity within each population. The study unveiled significant disparities in genetic diversity metrics between the two locations, Polymorphic diversity analysis further supported this, highlighting heightened genetic variation in Sample 2 from Thiruvananthapuram. Overall, Sample 2 consistently displayed elevated genetic diversity, suggesting a more robust genetic makeup compared to Sample 1 from Alleppey. These findings not only contribute to our understanding of the genetic variation, effective allele numbers, and information content within Sphagneticola trilobata populations but also hold implications for conservation strategies and evolutionary studies in the studied regions.

Sofia S*1 and Ajmila Majeed2 Click here to download file

Research Article | Published online : 08-May-2024